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calcium phosphate kit profection mammalian transfection system cat # e1200  (Promega)

 
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    Structured Review

    Promega calcium phosphate kit profection mammalian transfection system cat # e1200
    Calcium Phosphate Kit Profection Mammalian Transfection System Cat # E1200, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/profection+calcium+phosphate+transfection+kit/profection+mammalian+transfection+system/pm38621124-367-9-19
    Average 90 stars, based on 1 article reviews
    calcium phosphate kit profection mammalian transfection system cat # e1200 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Virus:

    Article Title: The Host Range of Gammaretroviruses and Gammaretroviral Vectors Includes Post-Mitotic Neural Cells
    Article Snippet: .. AZE-GFP virus and retroviral vector particles were produced by transient transfection of 293T cells using Profection calcium phosphate transfection kit (Promega, Madison, WI). ..

    Retroviral:

    Article Title: The Host Range of Gammaretroviruses and Gammaretroviral Vectors Includes Post-Mitotic Neural Cells
    Article Snippet: .. AZE-GFP virus and retroviral vector particles were produced by transient transfection of 293T cells using Profection calcium phosphate transfection kit (Promega, Madison, WI). ..

    Article Title: An exogenous retrovirus isolated from koalas with malignant neoplasias in a US zoo
    Article Snippet: .. A Profection calcium phosphate transfection kit (Promega) was used to transfect 293T cells by using plasmids encoding viral envelope (KoRV-A, KoRV-B, or GALV), Moloney MLV (MoMLV) gagpol , and a retroviral genome encoding β-gal as an indicator. ..

    Plasmid Preparation:

    Article Title: The Host Range of Gammaretroviruses and Gammaretroviral Vectors Includes Post-Mitotic Neural Cells
    Article Snippet: .. AZE-GFP virus and retroviral vector particles were produced by transient transfection of 293T cells using Profection calcium phosphate transfection kit (Promega, Madison, WI). ..

    Article Title: Development of measles virus-based shielded oncolytic vectors: suitability of other paramyxovirus glycoproteins
    Article Snippet: .. Briefly, 10μg of full-length DNA was transfected using the ProFection (Promega, Madison, WI) calcium phosphate transfection kit with 10 ng of the viral polymerase plasmid pEMC-La into 293-3-46 cells, which express the MV N and P proteins. ..

    Produced:

    Article Title: The Host Range of Gammaretroviruses and Gammaretroviral Vectors Includes Post-Mitotic Neural Cells
    Article Snippet: .. AZE-GFP virus and retroviral vector particles were produced by transient transfection of 293T cells using Profection calcium phosphate transfection kit (Promega, Madison, WI). ..

    Transfection:

    Article Title: The Host Range of Gammaretroviruses and Gammaretroviral Vectors Includes Post-Mitotic Neural Cells
    Article Snippet: .. AZE-GFP virus and retroviral vector particles were produced by transient transfection of 293T cells using Profection calcium phosphate transfection kit (Promega, Madison, WI). ..

    Article Title: Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere protein F
    Article Snippet: Briefly, siRNA duplexes were mixed with 2 μl Interferin (Autogen Bioclear) in media without serum and antibiotics, incubated for 15 minutess, then added to the cells with media containing serum and antibiotics. .. Mouse embryo fibroblasts Immortalised CDH1 +/+ and CDH1 −/− MEFs were cultured as previously described ( Garcia-Higuera et al., 2008 ). iMEFs were transiently transfected with the GFP-tagged constructs using ProFection calcium phosphate transfection kit (Promega) as per the manufacturers instructions. ..

    Article Title: Genetic Diversity of Koala Retroviral Envelopes
    Article Snippet: .. Transfection of 293T cells was carried out with ProFection Calcium Phosphate Transfection kit (Promega). ..

    Article Title: Influence of Naturally Occurring Simian Foamy Viruses (SFVs) on SIV Disease Progression in the Rhesus Macaque ( Macaca mulatta ) Model
    Article Snippet: .. 293T/17 cells (ATCC, Herndon, VA, USA) were transfected at 20%–25% confluence with SIV mac239 SpX cloned DNA (5 μg per 75 cm 2 flask) using Profection Calcium Phosphate Transfection Kit (Promega, Madison, WI, USA). ..

    Article Title: Development of measles virus-based shielded oncolytic vectors: suitability of other paramyxovirus glycoproteins
    Article Snippet: .. Briefly, 10μg of full-length DNA was transfected using the ProFection (Promega, Madison, WI) calcium phosphate transfection kit with 10 ng of the viral polymerase plasmid pEMC-La into 293-3-46 cells, which express the MV N and P proteins. ..

    Article Title: Low pH up‐regulates interleukin‐6 mRNA in L6‐G8C5 rat skeletal muscle cells independent of pH sensing by SNAT2(SLC38A2) transporters
    Article Snippet: .. After overnight incubation, the GM was discarded and 1 ml of fresh GM was added to the wells and the cells were transfected with anti‐SNAT2 SIL siRNA or SCR control using a Profection Calcium Phosphate Transfection Kit (Promega). ..

    Article Title: An exogenous retrovirus isolated from koalas with malignant neoplasias in a US zoo
    Article Snippet: .. A Profection calcium phosphate transfection kit (Promega) was used to transfect 293T cells by using plasmids encoding viral envelope (KoRV-A, KoRV-B, or GALV), Moloney MLV (MoMLV) gagpol , and a retroviral genome encoding β-gal as an indicator. ..

    Cell Culture:

    Article Title: Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere protein F
    Article Snippet: Briefly, siRNA duplexes were mixed with 2 μl Interferin (Autogen Bioclear) in media without serum and antibiotics, incubated for 15 minutess, then added to the cells with media containing serum and antibiotics. .. Mouse embryo fibroblasts Immortalised CDH1 +/+ and CDH1 −/− MEFs were cultured as previously described ( Garcia-Higuera et al., 2008 ). iMEFs were transiently transfected with the GFP-tagged constructs using ProFection calcium phosphate transfection kit (Promega) as per the manufacturers instructions. ..

    Construct:

    Article Title: Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere protein F
    Article Snippet: Briefly, siRNA duplexes were mixed with 2 μl Interferin (Autogen Bioclear) in media without serum and antibiotics, incubated for 15 minutess, then added to the cells with media containing serum and antibiotics. .. Mouse embryo fibroblasts Immortalised CDH1 +/+ and CDH1 −/− MEFs were cultured as previously described ( Garcia-Higuera et al., 2008 ). iMEFs were transiently transfected with the GFP-tagged constructs using ProFection calcium phosphate transfection kit (Promega) as per the manufacturers instructions. ..

    Clone Assay:

    Article Title: Influence of Naturally Occurring Simian Foamy Viruses (SFVs) on SIV Disease Progression in the Rhesus Macaque ( Macaca mulatta ) Model
    Article Snippet: .. 293T/17 cells (ATCC, Herndon, VA, USA) were transfected at 20%–25% confluence with SIV mac239 SpX cloned DNA (5 μg per 75 cm 2 flask) using Profection Calcium Phosphate Transfection Kit (Promega, Madison, WI, USA). ..

    Incubation:

    Article Title: Low pH up‐regulates interleukin‐6 mRNA in L6‐G8C5 rat skeletal muscle cells independent of pH sensing by SNAT2(SLC38A2) transporters
    Article Snippet: .. After overnight incubation, the GM was discarded and 1 ml of fresh GM was added to the wells and the cells were transfected with anti‐SNAT2 SIL siRNA or SCR control using a Profection Calcium Phosphate Transfection Kit (Promega). ..

    Control:

    Article Title: Low pH up‐regulates interleukin‐6 mRNA in L6‐G8C5 rat skeletal muscle cells independent of pH sensing by SNAT2(SLC38A2) transporters
    Article Snippet: .. After overnight incubation, the GM was discarded and 1 ml of fresh GM was added to the wells and the cells were transfected with anti‐SNAT2 SIL siRNA or SCR control using a Profection Calcium Phosphate Transfection Kit (Promega). ..



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    Promega calcium phosphate kit profection mammalian transfection system cat # e1200
    Calcium Phosphate Kit Profection Mammalian Transfection System Cat # E1200, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega profection calcium phosphate transfection kit
    Effect of siRNA silencing of expression of the SNAT2(SLC38A2) amino acid transporter on interleukin‐6 mRNA in L6‐G8C5 myoblasts. (A) L6 myoblasts were cultured in DMEM growth medium for 24 h and then transfected with 30 nM scrambled control siRNA (Scr) or silencing anti‐SNAT2 siRNA (Sil) for 16 h. Negative control cultures were also treated with <t>transfection</t> agent only, or no additions. The medium was then replaced with growth medium for 24 h and incubated for a further 6 h in MEM medium at pH 7.4 with 2 mM l ‐glutamine with 2% dialysed foetal bovine serum. Pooled data are shown from n = 4 independent experiments. (B, C) L6 myoblasts were transfected or treated with negative transfection controls as in (A) but the subsequent 6‐h incubations in MEM at pH 7.4 were performed either (B) in absence of ionomycin, or (C) with 0.5 µM ionomycin. Pooled data are shown from n = 4 independent experiments
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    Effect of siRNA silencing of expression of the SNAT2(SLC38A2) amino acid transporter on interleukin‐6 mRNA in L6‐G8C5 myoblasts. (A) L6 myoblasts were cultured in DMEM growth medium for 24 h and then transfected with 30 nM scrambled control siRNA (Scr) or silencing anti‐SNAT2 siRNA (Sil) for 16 h. Negative control cultures were also treated with transfection agent only, or no additions. The medium was then replaced with growth medium for 24 h and incubated for a further 6 h in MEM medium at pH 7.4 with 2 mM l ‐glutamine with 2% dialysed foetal bovine serum. Pooled data are shown from n = 4 independent experiments. (B, C) L6 myoblasts were transfected or treated with negative transfection controls as in (A) but the subsequent 6‐h incubations in MEM at pH 7.4 were performed either (B) in absence of ionomycin, or (C) with 0.5 µM ionomycin. Pooled data are shown from n = 4 independent experiments

    Journal: FASEB BioAdvances

    Article Title: Low pH up‐regulates interleukin‐6 mRNA in L6‐G8C5 rat skeletal muscle cells independent of pH sensing by SNAT2(SLC38A2) transporters

    doi: 10.1096/fba.2021-00088

    Figure Lengend Snippet: Effect of siRNA silencing of expression of the SNAT2(SLC38A2) amino acid transporter on interleukin‐6 mRNA in L6‐G8C5 myoblasts. (A) L6 myoblasts were cultured in DMEM growth medium for 24 h and then transfected with 30 nM scrambled control siRNA (Scr) or silencing anti‐SNAT2 siRNA (Sil) for 16 h. Negative control cultures were also treated with transfection agent only, or no additions. The medium was then replaced with growth medium for 24 h and incubated for a further 6 h in MEM medium at pH 7.4 with 2 mM l ‐glutamine with 2% dialysed foetal bovine serum. Pooled data are shown from n = 4 independent experiments. (B, C) L6 myoblasts were transfected or treated with negative transfection controls as in (A) but the subsequent 6‐h incubations in MEM at pH 7.4 were performed either (B) in absence of ionomycin, or (C) with 0.5 µM ionomycin. Pooled data are shown from n = 4 independent experiments

    Article Snippet: After overnight incubation, the GM was discarded and 1 ml of fresh GM was added to the wells and the cells were transfected with anti‐SNAT2 SIL siRNA or SCR control using a Profection Calcium Phosphate Transfection Kit (Promega).

    Techniques: Expressing, Cell Culture, Transfection, Control, Negative Control, Incubation